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目的 :检测孤啡肽 (nociceptin/orphaninFQ ,OFQ)和内吗啡肽 (endomorphins,EM )在神经源性痛模型大鼠疼痛相关脑区杏仁核、下丘脑、导水管中央灰质 (PAG)和纹状体的含量变化 ,以假手术大鼠为对照。方法 :利用大鼠L5/L6 脊神经结扎神经源性痛模型 ,采用放射免疫的方法 ,检测OFQ和内吗啡肽在疼痛相关脑区的变化。结果 :发现在下列脑区发生了显著变化 :(1 )内吗啡肽 1 (EM1 )含量在神经源性痛大鼠下丘脑较对照鼠增加了 38% ,纹状体降低了 41 % ,而在杏仁核和PAG与对照大鼠差异无显著性。(2 )内吗啡肽 2 (EM 2 )含量在神经源性痛大鼠PAG较对照大鼠增加了 778% ,在纹状体降低了 43 % ,而在杏仁核和下丘脑与对照大鼠差异无显著性。 (3)OFQ含量在在神经源性痛大鼠的杏仁核比对照大鼠增加了 841 % ,在PAG比对照大鼠增加了 459% ;而在下丘脑和纹状体与对照大鼠差异无显著性。结论 :神经源性痛引起中枢神经系统疼痛相关脑区EM 1、EM2和OFQ的含量发生改变。
OBJECTIVE: To detect the expression of nociceptin / orphaninFQ (OFQ) and endomorphins (EM) in the pain-associated amygdala, hypothalamus, aqueduct central gray matter (PAG) Body content changes to sham rats as a control. Methods: The changes of OFQ and endomorphin in pain-related brain regions were detected by radioimmunoassay using rat L5 / L6 spinal nerve ligation model. RESULTS: Significant changes were found in the following brain regions: (1) Endomorphin-1 (EM1) levels were increased by 38% in the hypothalamus of neuropathic pain rats and by 41% in the striatum, whereas in the There was no significant difference between amygdala, PAG and control rats. (2) The content of endomorphin-2 (EM2) in neuropathic pain rats increased by 778% in PAG and 43% in striatum compared with control rats, while in the amygdala and hypothalamus compared with control rats No significant. (3) OFQ content was increased 841% in the amygdala of neuropathic pain rats compared with the control rats, and 459% in the PAGs of the control rats; but no significant difference in the hypothalamus and striatum compared with the control rats Sex. CONCLUSION: Neurogenic pain causes changes in EM 1, EM 2 and OFQ in the brain regions associated with central nervous system pain.