CD3AK细胞是用小剂量CD3单抗交联CD3分子而活化的T细胞 ,它具有较强的细胞增殖和细胞毒效能。CD2 8分子则是T细胞激活过程中所需的协同刺激分子 ,可提供T细胞活化所需的第二信号。本文利用CD2 8单抗作为CD2 8分子的配体 ,观察了CD2 8信号途径的激活对CD3AK细胞的影响。结果显示CD2 8单抗可增强CD3AK细胞的增殖活性和趋化团聚形成集落的能力 ,并优先引起CD4+T细胞的增殖。 CD3AK cells are T cells activated by cross-linking CD3 molecules with low-dose CD3 monoclonal antibodies, which have strong cell proliferation and cytotoxicity. The CD28 molecule is a co-stimulatory molecule required for T cell activation and provides the second signal required for T cell activation. In this study, CD28 monoclonal antibody was used as a ligand of CD28, and the effect of activation of CD28 signal pathway on CD3AK cells was observed. The results showed that the CD28 monoclonal antibody can enhance the proliferative activity of CD3AK cells and the ability of chemotaxis aggregation to form colonies, and preferentially cause the proliferation of CD4+ T cells.