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GmSbh1(L13663)是大豆中被克隆的第一个同源异型盒(homeobox,HB)基因。该类基因在调控植物生长发育、响应逆境胁迫和激素诱导等方面发挥重要作用。本研究采用q RT-PCR技术,分析GmSbh1基因在大豆不同组织器官、叶片与种子发育、种子萌发以及响应高温高湿胁迫和激素诱导中的表达模式。结果表明,GmSbh1基因在大豆种子中的表达量高于幼苗,子叶和胚中的表达高于种皮,幼苗下胚轴、真叶和根中的表达量很低。大豆种子发育成熟过程中GmSbh1表达量逐渐上升,生理成熟期(R7期)表达量显著增加,完熟期(R8期)表达量最高;种子萌发过程中GmSbh1表达量呈先升后降趋势,吸胀24~48 h表达量上升,随后下降;随着大豆叶片的发育和衰老,GmSbh1表达呈先升后降趋势,功能叶期表达量最高。高温高湿胁迫后大豆种子中的GmSbh1表达量上升,胁迫48 h后上升的幅度显著增大;与对照相比,不抗田间劣变品种宁镇1号显著上调表达,抗性品种湘豆3号下调表达。100μmol/L ABA、100μmol/L Me JA、100μmol/L GA3和20μmol/L IAA处理宁镇1号1 h后,GmSbh1的表达量显著升高,随着处里时间的延长,表达量降低;同样浓度的几种激素处里湘豆3号后,GmSbh1表达变化的规律不明显。以上结果表明,GmSbh1基因可能参与大豆种子与叶片的发育,响应高温高湿胁迫和激素诱导。
GmSbh1 (L13663) is the first homeobox (HB) gene cloned in soybean. These genes play an important role in the regulation of plant growth and development, response to stress and hormone-induced stress. In this study, q RT-PCR was used to analyze the expression patterns of GmSbh1 gene in different tissues and organs of soybean, leaf and seed development, seed germination, and response to high temperature and high humidity stress and hormone induction. The results showed that the expression level of GmSbh1 gene in soybean seeds was higher than that in seedlings, the expression in the cotyledons and embryos was higher than that in the seed coat, and the expression level of GmSbh1 in seedlings was very low. The expression of GmSbh1 gradually increased during the maturation of soybean seeds, the expression of GmSbh1 in physiological maturity (R7) increased significantly, while the expression of GmSbh1 in maturity (R8) was the highest; the expression of GmSbh1 increased first and then decreased, The expression of GmSbh1 increased firstly and then decreased with the development and senescence of soybean leaves. The expression level of functional GmSbh1 was the highest at 24-48 h. The expression of GmSbh1 in soybean seeds increased under high temperature and high humidity stress, and increased significantly after 48 h of stress. Compared with the control, Ningmai 1, Number down expression. After treated with 100μmol / L ABA, 100μmol / L Me JA, 100μmol / L GA3 and 20μmol / L IAA for 1 h, the expression of GmSbh1 was significantly increased. Concentrations of several hormones in Xiangdou 3, GmSbh1 expression changes in the law is not obvious. The above results showed that GmSbh1 gene may be involved in the development of soybean seeds and leaves, in response to high temperature and high humidity stress and hormone induction.