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目的 研究严重急性呼吸综合征(SARS)患者血清中抗SARS冠状病毒(SARS CoV)核壳蛋白(N)及刺突蛋白(S)S1区特异性抗体的产生规律,评价这两种蛋白在SARS诊断研究中的作用。方法 采用ELISA法检测86例确诊SARS患者血清中针对N和S1蛋白IgG(N- IgG和S1 -IgG),并与SARS- CoVIgG水平进行比较。结果 两种蛋白的特异性抗体的阳性率均随着病程的延长而增高。N -IgG在发病第1周检出阳性率为14% (6 /44),第2周为56% (10 /18),第3周达到100% (24 /24);S1- IgG第1周检出阳性率为5% ( 2 /44 ),第2周为39% ( 7 /18 ),第3周阳性率达到83% ( 20 /24)。两种蛋白抗体的检测结果分别与SARS- CoVIgG结果比较,检测符合率分别为88% (76 /86)和83% (71 /86 )。结合免疫印迹和ELISA两种方法检出正常人群SARS CoVN -IgG阳性率为1. 88%(14 /745)。结论 SARS -CoVN和S1重组蛋白对于SARS诊断试剂的研究具有重要价值。
Objective To study the production of anti-SARS coronavirus (SARS CoV) nucleocapsid protein (N) and spike protein (S1) specific antibodies in serum of patients with severe acute respiratory syndrome (SARS) The role of diagnostic research. Methods The serum levels of N and S1 IgG (N-IgG and S1-IgG) in 86 patients with confirmed SARS were detected by ELISA and compared with that of SARS-CoVIgG. Results The positive rates of the specific antibodies of the two proteins increased with the duration of the disease. The positive rate of N-IgG was 14% (6/44) in the first week, 56% (10/18) in the second week, and 100% (24/24) in the third week. The positive rate of S1- The positive rate was 5% (2/44) in the second week, 39% (7/18) in the second week and 83% (20/24) in the third week. The detection results of the two protein antibodies were respectively compared with the results of SARS-CoVIgG, and the coincidence rates were 88% (76/86) and 83% (71/86) respectively. 88% (14/745). The positive rate of SARS CoVN-IgG in normal population was detected by Western blot and ELISA. Conclusion The SARS-CoVN and S1 recombinant proteins are of great value in the research of SARS diagnostic reagents.