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目的:优选大孔吸附树脂富集纯化牡丹皮中丹皮总苷的最佳工艺参数。方法:采用HPLC法测定丹皮总苷含量,考察D101、D201、D301、D401、AB-8、NKA-96种大孔树脂对芍药苷的吸附和解吸附性能,并进一步考察分离纯化条件。结果:D101大孔树脂对丹皮总苷提取液纯化最优,上样液芍药苷浓度为0.83mg·mL-1、pH6.5、洗脱流速1BV·h-1,分离纯化条件为:先用10BV蒸馏水洗脱,然后用4BV70%乙醇洗脱,收集70%乙醇洗脱液,浓缩、干燥,即得到丹皮总苷。结论:D101大孔树脂可用于牡丹皮水提取液中丹皮总苷的富集纯化。
Objective: To optimize the macroporous resin enrichment of the purified Cortex Moutan best glycosides of the best technical parameters. Methods: HPLC method was used to determine the content of paeoniflorin. The adsorption and desorption properties of paeoniflorin from D101, D201, D301, D401, AB-8 and NKA-96 macroporous resins were investigated, and the isolation and purification conditions were further investigated. Results: D101 macroporous resin was the best for the purification of total glycosides of Radix Bupleuri. The concentration of paeoniflorin in the sample was 0.83 mg · mL-1, pH6.5, and the elution flow rate was 1BV · h-1. The isolation and purification conditions were as follows: Elute with 10BV distilled water, and then use 4BV70% ethanol to elute, collect 70% ethanol eluent, concentrate and dry, to obtain the total glycosides of Panda. Conclusion: D101 macroporous resin can be used to enrich and purify total glycoside of Paeonia osmanthus in the water extract of Cortex Moutan.