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目的:建立以高效液相色谱法测定消核灵胶囊延胡索乙素的含量的方法。方法:采用C18色谱柱(4.6 mm×150mm,5μm);流动相为乙腈-2%冰醋酸溶液(用三乙胺调pH 5.0)(25∶75);检测波长280 nm,流速1 mL.min-1,柱温为30℃。结果:延胡索乙素的检测浓度在44.68～357.44 mg.L-1与峰面积积分值呈良好线性关系(r=0.999 9,n=5);平均回收率为101.10%,RSD 2.42%(n=5)。结论:本法简便、快速、准确,可用于消核灵胶囊的质量控制。
Objective: To establish a method for the determination of tetrahydropalmatine in Xiaojianling capsules by high performance liquid chromatography. Methods: C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was acetonitrile-2% acetic acid solution (pH 5.0 with triethylamine) (25:75). The detection wavelength was 280 nm and the flow rate was 1 mL.min -1, the column temperature is 30 ℃. Results: The calibration curve of tetrahydropalmatine was in the range of 44.68 ~ 357.44 mg.L-1 with a good linear relationship (r = 0.999 9, n = 5). The average recovery was 101.10% and the RSD was 2.42% 5). Conclusion: This method is simple, rapid and accurate and can be used for the quality control of Capsules.