论文部分内容阅读
目的:探讨桑叶黄酮对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:小鼠随机分为空白组,模型组,联苯双酯100 mg.kg-1组、桑叶黄酮200,400,800 mg.kg-1组,连续ig给药14 d,1次/d。电镜观察CCl4急性肝损伤模型小鼠肝组织形态学变化,分光光度法测定小鼠血清中门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT),肝组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量。结果:桑叶黄酮能显著降低CCl4所致小鼠血清ALT和AST活性的升高(P<0.01),并能显著升高SOD和GSH-Px活性(P<0.01),降低MDA含量(P<0.01),保护肝细胞。结论:桑叶黄酮对CCl4诱导的小鼠肝损伤有很强的保护作用。
Objective: To investigate the protective effect of mulberry leaf flavonoids on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: The mice were randomly divided into blank group, model group, bifendate 100 mg.kg-1 group, mulberry leaf flavonoids 200,400,800 mg.kg-1 group, continuous ig administration for 14 days, once a day. Electron microscopy was used to observe the morphological changes of liver tissue in CCl4-induced acute liver injury model mice. The serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured by spectrophotometry. The levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content. Results: Mulberry flavonoids could significantly reduce the serum ALT and AST activity of mice induced by CCl4 (P <0.01), and significantly increase the activity of SOD and GSH-Px (P <0.01) and decrease the content of MDA (P < 0.01), protect liver cells. Conclusion: Mulberry flavonoids have a strong protective effect on CCl4-induced liver injury in mice.