论文部分内容阅读
目的用~(99m)Tc-亚甲基二膦酸盐(~(99m)Tc-MDP)联合胶体磷酸铬~(32)P治疗大鼠佐剂型关节炎,探讨其疗效并与单一使用~(99m)Tc-MDP或胶体磷酸铬~(32)P治疗进行比较。方法利用佐剂诱导SD大鼠的关节炎模型(AA),给予胶体磷酸铬~(32)P踝关节腔内注射以及~(99m)Tc-MDP腹腔注射,在不同的时间点观察大鼠左踝关节的左右径宽度、血清肿瘤坏死因子(TNF)和白细胞介素-1β(IL-1β)水平及关节病理的变化。结果联合治疗组大鼠左踝关节左右径宽度较~(32)P胶体治疗组小[第4周(7.11±0.34)mmvs(7.57±0.29)mm,P<0.01]。联合治疗较~(32)P胶体治疗更能有效地降低血清TNF[第4周时(1.6±0.4)ng/mlvs(2.0±0.3)ng/M,P<0.05]和IL-1β[第4周时(0.271±0.033)ng/mlvs(0.308±0.024)ng/ml,P<0.05;第6周时(0.209±0.023)ng/mlvs(0.255±0.016)ng/ml,P<0.01]的水平。在病理检查上,联合治疗组滑膜增生程度较~(99m)Tc-MDP治疗组轻,而炎细胞浸润程度较~(32)P胶体治疗组轻。结论~(99)Tc-MDP联合胶体磷酸铬~(32)P治疗大鼠佐剂型关节炎较单用~(99m)Tc-MDP或胶体磷酸铬~(32)P治疗效果更好。
OBJECTIVE To investigate the therapeutic effect of adjuvant arthritis in rats with ~ (99m) Tc-methylene diphosphonate (~ (99m) Tc-MDP) and colloidal chromium (32) 99m) Tc-MDP or colloidal chromium (32) P treatment. Methods The arthritis model (AA) of SD rats was induced by adjuvant. The rats were injected intraperitoneally with colloidal chromium (32) P ankle and intraperitoneal injection of 99m Tc-MDP. At different time points, rats were observed Left and right width of ankle joint, serum tumor necrosis factor (TNF) and interleukin-1β (IL-1β) levels and joint pathological changes. Results The left and right width of the left ankle joint in the combined treatment group was smaller than that in the colloidal group ([7.11 ± 0.34] mm vs (7.57 ± 0.29) mm in the 4th week, P <0.01]. Combination therapy was more effective in lowering serum TNF (1.6 ± 0.4 ng / ml vs (2.0 ± 0.3) ng / M, P <0.05] and IL-1β [Week 4 Ml at week 6 (0.271 ± 0.033) ng / mlvs (0.308 ± 0.024) ng / ml, P <0.05; . In the pathological examination, synovial hyperplasia in the combination therapy group was lighter than that in the 99m Tc-MDP group, while inflammatory cell infiltration was lighter than that in the (32) P colloid treatment group. Conclusion The treatment of adjuvant arthritis with ~ (99) Tc-MDP and colloidal chromium (32) P treatment is better than 99m Tc-MDP alone or colloidal chromium 32 P treatment.