论文部分内容阅读
目的 检测IL-33及其可溶性受体ST2(sST2)在非节段型白癜风患者外周血的表达,探讨其临床意义.方法 应用酶联免疫吸附法(ELISA)分别检测进展期28例、稳定期26例和30名健康对照者血清中IL-33和sST2蛋白水平;采用SYBR Green实时荧光定量RT-PCR方法检测不同时期白癜风患者及对照组外周血单个核细胞(PBMC)中IL-33 mRNA和sST2mRNA的转录水平.结果 非节段型白癜风患者外周血中IL-33和sST2的表达量在进展期和稳定期均高于健康对照组,除稳定期IL-33表达量与对照组差异无统计学意义外(P ＞0.05),其余差异均有统计学意义(P＜0.05);非节段型白癜风进展期及稳定期患者PBMC中IL-33 mRNA和sST2 mRNA的表达量均显著高于健康对照组,差异均有统计学意义(P＜0.05).结论 外周血中IL-33和sST2的异常表达可能与非节段型白癜风的发病有密切相关性.“,”Objective To detect the expression level of cytokines interleukin( IL)-33 and its soluble receptor ST2 ( sST2 ) in peripheral blood of patients with non-segmental vitiligo,and to explore their clinical significance. Methods The levels of IL-33 and sST2 in the serum of patients with progressive(n =28) and stable(n =26) vitiligo and 30 healthy human controls were detected by enzyme-linked immunosorbent assay (ELISA). The expression of IL-33 mRNA and sST2 mRNA in peripheral blood mononuclear cells( PBMC) in patients at different stages and those in the control group was detected by SYBR Green reverse transcriotion-polymerase chain reaction ( RT-PCR). Results The expression levels of serum IL-33 and sST2 were higher in peripheral blood of patients in progressive stage and stable stage of non-segmental vitiligo than in the normal controls, and the difference was statistically significant ( P ＜ 0.05 ). The expression of IL-33 was not significantly different in stable stage(P ＞ 0.05), but there was remarkable difference in other stages,as compared with the control group (P ＜0.05). The patients with non-segmental vitiligo had a higher serum level of IL-33 mRNA and sST2 mRNA in progressive and stable stages,as compared with the healthy controls,and the difference was statistically significant ( P ＜ 0. 05 ). Conclusion There may be a close association between the abnormal expression of serum IL-33 and sST2 and non-segmental or generalized vitiligo.