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目的观察油酸致小鼠早期急性肺损伤(ALI)中血管内皮细胞生长因子(VEGF)表达的变化。方法48只BALB/c小鼠随机分为两组,每组24只。ALI组从尾静脉注射油酸0.9ml/kg制备ALI模型;正常对照组注入等量生理盐水。8h后每组随机抽半数小鼠处死后行肺泡灌洗(BAL),另一半小鼠右肺称重后烘干,左肺留取病理标本;分别测定肺湿/干质量比、肺泡灌洗液(BALF)总细胞计数、中性粒细胞计数、蛋白含量变化;ELISA测定血清和BALF中白细胞介素6(IL-6)、VEGF的含量;免疫组化法检测组织中VEGF表达的变化。结果ALI组肺湿/干质量比、BALF中蛋白含量和总细胞计数、中性粒细胞计数、血清和BALF中IL-6含量、血清VEGF含量与正常对照组比较均明显升高,ALI组BALF中VEGF的含量和肺组织中VEGF表达积分吸光度值(IA)较正常对照组均明显降低,以上比较的差异均有统计学意义(均P<0.05)。结论小鼠早期ALI血清VEGF表达升高而BALF和肺组织中VEGF表达降低。
Objective To observe the changes of vascular endothelial growth factor (VEGF) expression in early acute lung injury (ALI) induced by oleic acid in mice. Methods 48 BALB / c mice were randomly divided into two groups of 24 rats. The rats in ALI group were injected with 0.9ml / kg oleic acid to prepare ALI model. The normal control group was injected with normal saline. After 8h, half of mice in each group were randomly sacrificed and BALF was sacrificed. The other half of the mice were weighed and dried. The left lungs were taken for pathological examination. The lung wet / dry weight ratio, alveolar lavage (BALF). The levels of IL-6 and VEGF in serum and BALF were detected by ELISA, and the changes of VEGF expression were detected by immunohistochemistry. Results The lung wet / dry weight ratio, BALF protein content and total cell count, neutrophil count, IL-6 level in serum and BALF, serum VEGF level in ALI group were significantly higher than those in normal control group The content of VEGF and the integral expression of VEGF in lung tissue (IA) were significantly lower than the normal control group, the above differences were statistically significant (P <0.05). Conclusion The expression of VEGF in early ALI serum of mice increased and the expression of VEGF in BALF and lung decreased.