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目的 研究VitaminC (VC)拮抗三氧化二镍的细胞毒作用及机理。方法 采用体外细胞培养法测定在维生素C (2 5 ,5 0 ,10 0 μmol/L) )作用下 ,体外染镍肺泡巨噬细胞 (alveolarmacrophage ,AM )的存活率及活力。同时观察细胞内一氧化氮 (NO)和活性氧的产生 ,以及一氧化氮合酶 (NOS)活力的变化。结果 在体外染镍肺泡巨噬细胞中加入不同浓度的维生素C后可提高该细胞的存活率和活力 ,可减少NO和活性氧的产生 ,降低NOS和活性 ,其中以10 0 μmol/L的维生素C最为明显。 结论 镍可致细胞脂质过氧化 ,维生素C具有拮抗镍对肺泡巨噬细胞的细胞毒作用 ,可能与抗氧化功能有关
Aim To study the cytotoxicity and mechanism of vitamin C (VC) antagonizing nickel (II) oxide. Methods Survival rate and viability of alveolar macrophages (AMs) infected by vitamin C (25, 50, 100 μmol / L) were determined by in vitro cell culture. The changes of nitric oxide (NO) and reactive oxygen species (ROS) and the activity of nitric oxide synthase (NOS) were also observed. Results The addition of different concentrations of vitamin C to nickel-alveolar macrophages in vitro could increase the viability and viability of the cells, reduce the production of NO and reactive oxygen species, and decrease the activity of NOS. Among them, vitamins with a concentration of 100 μmol / L C is the most obvious. Conclusion Nickel can lead to cellular lipid peroxidation. Vitamin C can inhibit the cytotoxic effect of nickel on alveolar macrophages, which may be related to the anti-oxidative function