论文部分内容阅读
目的:了解组织工程修复及不同加力时间点对正畸移动牙牙周骨形成蛋白BMP表达的影响。方法:应用牙颈部结扎、高糖高粘饮食配合牙石植入法建立角形缺损慢性牙周炎新西兰大白兔动物模型,并随机分成两组:20 d加力组、40 d加力组;使用壳聚糖磷酸钙/PRP/骨髓基质干细胞复合物组织工程材料结合GTR技术修复牙槽骨缺损;分别于修复术后20 d和40 d开始正畸加力。另设正常牙周对照组,以相同条件正畸加力。加力30 d后处死实验动物,使用LSAB免疫组化法及图像分析仪对各组进行牙周BMP分布灰度测量分析。结果:20 d加力组BMP平均灰度值为4.522±0.057,40 d加力组为4.321±0.071,正常对照组为3.372±0.051,差异具有统计学意义。结论:组织工程修复术后正畸移动牙的牙周BMP表达高于正常对照组,术后20 d加力组牙周BMP表达高于40 d加力组。
OBJECTIVE: To investigate the effect of tissue engineering repair and different time points of force application on BMP expression of periodontal bone morphogenetic protein in orthodontic tooth. Methods: New Zealand white rabbits with chronic periodontitis were established by ligating the tooth neck and high-sugar and high-viscosity diet with tartar implantation. The animals were randomly divided into two groups: 20-day plus 40-day plus-force group; Chitosan / calcium phosphate / PRP / BMSCs tissue engineering materials combined with GTR technique were used to repair alveolar bone defects. Orthodontic force was applied 20 days and 40 days after repair respectively. Another normal periodontal control group, with the same conditions of orthodontic force. After 30 days of force application, the experimental animals were sacrificed and the periodontal BMP distributions were measured by LSAB immunohistochemistry and image analyzer. Results: The average gray value of BMP in 20-day addictive group was 4.522 ± 0.057, while in addictive group was 4.321 ± 0.071 on day 20 and 3.372 ± 0.051 in normal control group, the difference was statistically significant. Conclusion: The expression of BMP in periodontal tissue of orthodontic tooth after tissue engineering repair is higher than that of normal control group.