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以亲本水稻为对照,应用13C脉冲标记和磷脂脂肪酸技术,分析转Bt基因对水稻根际微生物多样性的影响.结果表明:转Bt基因水稻与亲本水稻根际均以饱和脂肪酸和支链脂肪酸为主,单不饱和脂肪酸次之,多不饱和脂肪酸最少.苗期、拔节期和抽穗期,转Bt基因水稻根际革兰氏阳性菌(G+)代表性磷脂脂肪酸含量显著低于亲本水稻;革兰氏阴性菌(G-)代表性磷脂脂肪酸含量显著高于亲本水稻.水稻各生育期,转Bt基因未对水稻根际土壤真菌、放线菌磷脂脂肪酸含量造成显著影响,且转Bt基因水稻与亲本水稻根际微生物磷脂脂肪酸13 C含量无显著性差异.表明外源Bt基因插入仅对水稻根际微生物多样性造成短暂影响,不具有持续性.
The parental rice was used as control and the effects of transgenic Bt gene on rhizosphere microbial diversity were analyzed by 13C pulse labeling and phospholipid fatty acid assay.The results showed that both rhizosphere and saturated fatty acid and branched-chain fatty acids In the seedling stage, jointing stage and heading stage, the content of representative phospholipid fatty acids in G + of Bt transgenic rice was significantly lower than that of the parental rice The content of representative phospholipid fatty acid of G- was significantly higher than that of the parental rice.During the growth period of rice, transgenic Bt gene had no significant effect on the content of phospholipid fatty acids in rice rhizosphere soil and actinomycete, There was no significant difference in the 13 C content of microbial phospholipid fatty acid between the parental rice and rhizosphere soil, indicating that the insertion of exogenous Bt gene only had a short-term impact on rhizosphere microbial diversity of rice without persistence.