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为确定丁香叶黄酮的保肝作用,将小鼠随机分为5组,即对照组、模型组、丁香叶黄酮高剂量组(40 mg/kg体重)、中剂量组(35 mg/kg体重)、低剂量组(30 mg/kg体重),以血清转氨酶(ALT、AST)、肝组织指标(MDA、NO、SOD、GSH、GSH-Px)及II相药物代谢酶GSTA1为检测对象,结合小鼠肝脏病理变化综合分析。结果显示,丁香叶黄酮高剂量组血清转氨酶和肝组织中MDA、NO均极显著降低(P<0.01),SOD、GSH、GSH-Px均极显著升高(P<0.01),GSTA1的含量也极显著升高(P<0.01);肝脏组织病理学显示脂肪变性、坏死及炎性细胞浸润减轻;而中、低剂量组的各指标和组织学变化均不如高剂量组明显。研究表明,丁香叶黄酮以40 mg/kg体重剂量对小鼠药物性肝损伤有较好的保护作用。
To determine the hepatoprotective effect of clove leaf flavonoids, the mice were randomly divided into 5 groups: control group, model group, clove leaf flavone high dose group (40 mg / kg body weight), medium dose group (35 mg / kg body weight) , Low dose group (30 mg / kg body weight) and serum transaminase (ALT, AST), liver tissue index (MDA, NO, SOD, GSH, GSH-Px) and phase II drug metabolizing enzyme GSTA1 Comprehensive analysis of liver pathological changes in rats. The results showed that the content of MDA and NO in the serum aminotransferase and liver tissue were significantly decreased (P <0.01), the contents of SOD, GSH and GSH-Px were significantly increased (P <0.01), and the contents of GSTA1 (P <0.01). Histopathology of the liver showed steatosis, necrosis and inflammatory cell infiltration alleviation. However, the indexes and histological changes in the medium and low dose groups were not as obvious as those in the high dose group. Studies have shown that clove leaf flavonoids at a dose of 40 mg / kg mice with drug-induced liver injury have a good protective effect.