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目的:通过研究喉咽鳞状细胞癌组织与癌旁组织中唐氏综合征侯选区域1(DSCR1)基因的表达,了解其表达与肿瘤临床特征的关系,探讨DSCR1基因对喉咽鳞状细胞癌的生物学行为的影响。方法:免疫组织化学p-V9000法,使用兔抗DSCR1抗体DCT3,检测DSCR1基因蛋白在喉咽鳞状细胞癌与正常癌旁组织中的表达,并将其与临床资料之间进行统计学分析。结果:肿瘤组织中DSCR1基因表达阳性率94.9%,癌旁组织中表达阳性率35.9%,差异有统计学意义(t=23.69,P<0.01);DSCR1基因的表达在不同的病理分化程度、TNM分期差异有统计学意义(P<0.05);DSCR1基因的表达在不同的肿瘤发病年龄、性别、部位、生长方式、淋巴结转移以及吸烟史组均差异无统计学意义。结论:DSCR1基因在喉咽鳞状细胞癌的发生、发展过程中有重要作用,可以影响肿瘤的生物学行为。
OBJECTIVE: To investigate the relationship between the expression of DSCR1 gene and clinical features of tumor in squamous cell carcinoma of the hypopharyngeal squamous cell carcinoma and its adjacent tissues, and to explore the role of DSCR1 gene in regulating the expression of DSCR1 gene in hypopharyngeal squamous cell The biological effects of cancer. Methods: Immunohistochemistry p-V9000 method was used to detect the expression of DSCR1 gene in squamous cell carcinoma of the larynx pharynx and adjacent normal tissues using rabbit anti-DSCR1 antibody DCT3, and statistical analysis was made between it and the clinical data. Results: The positive rate of DSCR1 gene was 94.9% in tumor tissues and 35.9% in adjacent non-cancerous tissues (t = 23.69, P <0.01). The expression of DSCR1 gene in different pathological differentiation, TNM The difference of staging was statistically significant (P <0.05). There was no significant difference in the expression of DSCR1 gene between different age, gender, location, growth pattern, lymph node metastasis and smoking history. Conclusion: The DSCR1 gene plays an important role in the carcinogenesis and progression of hypopharyngeal squamous cell carcinoma and may affect the biological behavior of the tumor.