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目的采用响应面法优化川丹参中总黄酮提取工艺,并初步探究总黄酮的体外抗氧化活性。方法在单因素试验的基础上,以甲醇浓度(A)、提取时间(B)、提取温度(C)为自变量,运用Box-Behnken试验设计-响应面法优化超声辅助提取川丹参总黄酮工艺。同时,测定川丹参总黄酮对超氧自由基(O2-·)、羟基自由基(·OH)和DPPH·3种自由基的体外清除活性。结果通过响应面试验设计得到川丹参总黄酮最佳提取工艺:甲醇浓度71%,超声时间52 min,超声温度55℃,在此工艺条件下提取得率可达12.493%。在所测浓度范围内,川丹参总黄酮对O2-·、·OH和DPPH·均有一定的清除效果,其最大清除率分别达到93.4%,60.3%和40.3%。结论 Box-Behnken设计-响应面法优化川丹参总黄酮提取工艺方法简便,可预测性较好;川丹参总黄酮具有体外抗氧化活性,值得进一步研究和利用。
Objective To optimize the extraction technology of total flavonoids from Radix Salviae Miltiorrhizae by response surface methodology and investigate the antioxidant activity of flavonoids in vitro. Methods Based on the single factor experiment, the concentration of methanol (A), extraction time (B) and extraction temperature (C) were used as independent variables. The Box-Behnken design was used to optimize the ultrasound-assisted extraction of total flavonoids from Radix Salviae Miltiorrhizae . At the same time, the total flavonoids of Radix Salviae Miltiorrhizae were assayed for the in vitro scavenging activity on the free radical (O2- ·), hydroxyl radical (· OH) and DPPH · 3 free radicals. Results The optimum extraction process of total flavonoids from Radix Salviae Miltiorrhizae was obtained through the response surface design experiment. The optimum extraction conditions were as follows: methanol concentration 71%, ultrasonic time 52 min, ultrasonic temperature 55 ℃. The extraction yield reached 12.493% under this condition. In the tested concentration range, total flavonoids of Radix Salviae Miltiorrhizae had certain scavenging effects on O2- ·, · OH and DPPH · with the maximum clearance rates reaching 93.4%, 60.3% and 40.3% respectively. Conclusion Box-Behnken design-response surface methodology is a simple and predictable method to optimize the extraction of total flavonoids from Radix Salviae Miltiorrhizae. The total flavonoids of Radix Salviae Miltiorrhizae are in vitro antioxidant activity and deserve further study.