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[Objective]To explore inhibiting effect of 12 heat-clearing and detoxicating drugs originated in Guangdong on the expression of EBV viral capsid antigen in cells in vitro and the cytotoxic effect of which on B95-8cell. [Methods]Study the inhibiting effect of 12 traditional Chinese medicines on the expression of capsid antigen in B95-8cell by indirect immunoenzyme technique Observe the cytotoxic effect of traditional Chinese medicines on B95-8cell cultured under stimulating circumstances by trypan blue exclusion test. [Results]The expression of VCA in B95-8cells cultured under stimulating circumstances could be significantly inhibited by 12 heat-clearing and detoxicating drugs originated in Guangdong. Most of the medicines were dose-dependent. The dose of corresponding drug increased 1 000 times,the 12 traditional Chinese medicines had significant cytotoxic effect on B95-8cells and their effects were concentration-dependent. [Conclusions]The inhibiting effect of12 heat-clearing and detoxicating drugs originated in Guangdong on the expression of EBV viral capsid antigen was significant and with high concentrations,they all had cytotoxic effect on B95-8cells.
[Objective] To explore inhibiting effect of 12 heat-clearing and detoxicating drugs originated in Guangdong on the expression of EBV viral capsid antigen in cells in vitro and the cytotoxic effect of which on B95-8 cell. [Methods] Study of inhibiting effect of 12 traditional Chinese medicines on the expression of capsid antigen in B95-8cell by indirect immunoenzyme technique Observe the cytotoxic effect of traditional Chinese medicines on B95-8cell cultured under stimulating circumstances by trypan blue exclusion test. [Results] The expression of VCA in B95-8 cells cultured under stimulating circumstances could be significantly inhibited by 12 heat-clearing and detoxicating drugs originated in Guangdong. Most of the medicines were dose-dependent. The dose of corresponding drugs increased 1,000 times, the 12 traditional Chinese medicines had significant cytotoxic effect on B95 -8cells and their effects were concentration-dependent. [Conclusions] The inhibiting effect of12 heat-clearing and deto xicating drugs originated in Guangdong on the expression of EBV viral capsid antigen was significant and with high concentrations, they all had cytotoxic effect on B95-8 cells.