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目的研究孕期多环芳烃暴露对新生儿神经行为发育的影响以及胎盘P300的变化,了解其是否可以作为一种效应标志物。方法收集孕产妇的尿液及其相应的胎盘(胎儿面)组织,用高效液相色谱法(HPLC)和酶联免疫吸附测定(ELISA)法分别测定尿中1-羟基芘和胎盘中P300的含量,运用20项神经行为测试法(NBNA)测定新生儿神经行为功能,计算NBNA总分,比较两地胎盘中P300含量的差异。结果共有116例新生儿胎盘组织符合条件,太原组和长治组的P300结果差异有统计学意义(P<0.05),太原组P300含量高于长治组,P300含量与尿中1-羟基芘含量呈正相关(r=0.202,P<0.05)。结论 P300的含量与孕期多环芳烃暴露对新生儿神经行为发育的影响有关联,但P300作为孕期多环芳烃暴露对新生儿神经行为发育造成影响的特定生物标志物仍需进一步研究。
Objective To study the effects of prenatal exposure to PAHs on neurobehavioral development and the changes of placental P300 in order to find out whether PAH could be an effective marker. Methods Urine of pregnant women and their corresponding placenta (fetus) tissues were collected. The urinary levels of 1-hydroxypyrene and P300 in the placenta were determined by HPLC and ELISA, respectively. 20 neurobehavioral tests (NBNA) were used to determine the neonatal neurobehavioral function. The total score of NBNA was calculated. The difference of P300 content in placenta between the two groups was compared. Results A total of 116 cases of neonatal placenta accords with the conditions. There was significant difference in P300 between Taiyuan group and Changzhi group (P <0.05). The content of P300 in Taiyuan group was higher than that in Changzhi group. P300 content was positively correlated with urinary 1-hydroxy pyrene content (R = 0.202, P <0.05). Conclusions P300 levels are correlated with the effects of polycyclic aromatic hydrocarbons (PAHs) exposure during pregnancy on the neurobehavioral development in neonates. However, specific biomarkers of P300, which may affect the neurobehavioral development of newborns during pregnancy, need to be further studied.