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目的 :利用COS7细胞表达角质细胞生长因子 (KGF)并检测其活性。方法 :利用脂质体把KGF基因转入COS7细胞中 ,再用Western印迹和ELISA方法检测KGF蛋白的表达 ,然后用MTT法检测其活性。结果 :KGF基因在COS7中得到了表达 ,并对小鼠肠上皮细胞 (IEC_6 )具有刺激增殖作用。结论 :KGF有望成为肠型放射病治疗的新型药物。
Objective: To express keratinocyte growth factor (KGF) using COS7 cells and test its activity. Methods: The KGF gene was transfected into COS7 cells by liposome. The expression of KGF protein was detected by Western blotting and ELISA. The activity of KGF protein was detected by MTT assay. Results: KGF gene was expressed in COS7 and stimulated proliferation of mouse intestinal epithelial cells (IEC_6). Conclusion: KGF is expected to be a new drug for the treatment of enteropathy.