目前有研究发现一氧化氮(nitric oxide,NO)与顺铂导致的内耳毒性关系密切,增加的NO可能参与了顺铂的耳毒性作用。近来又有文献报道,丹参salvia miltiorrhiza)可以通过降低一氧化氮合酶(nitric oxide synthase,NOS)活性以降低庆大霉素耳毒性。但是,丹参能否降低顺铂耳毒性,是否通过影响NOS表达来实现,目前尚未见报道。本研究拟采用顺铂内耳中毒的动物模型,通过免疫组织化学、图像分析、听性脑干反应测定、电镜观察等方法,来探讨顺铂耳蜗毒性的机制,以期寻求防治顺铂耳蜗毒性损伤的有效药物。 It has been found that nitric oxide (NO) is closely related to cisplatin-induced toxicity in the inner ear. Increased NO may be involved in the ototoxicity of cisplatin. Recently, it has been reported in the literature that Salvia miltiorrhiza can reduce gentamicin ototoxicity by decreasing the activity of nitric oxide synthase (NOS). However, Salvia can reduce cisplatin ototoxicity, whether through the impact of NOS expression to achieve, has not been reported. This study intends to use cisplatin poisoning in animal models of anoxia, immunohistochemistry, image analysis, auditory brainstem response, electron microscopy and other methods to explore cisplatin cochlear toxicity in order to seek prevention and treatment of cisplatin cochlear toxicity Effective drug.