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通过应用核素法测定糖尿病胃瘫大鼠的胃排空 ,建立了糖尿病胃瘫大鼠的模型 ;据此模型观察了小剂量红霉素 (3mg kg)对糖尿病胃瘫大鼠液相胃排空的影响。结果显示该药能够显著减小其胃排空的一小时残留率 (R1H) [(6 3%± 6 .8% )vs.(36 %± 3.8% ) ];但此作用具有显著个体差异 ,根据所设标准分成红霉素有效组 (E组 )和无效组 (F组 )后 ,发现两组疗效具有显著差异。应用受体相关基因芯片初步筛选了这两组大鼠胃窦组织的差异表达基因。结果筛选出 10条差异表达基因 ,其中对多巴胺受体D3和神经肽Y受体Y5基因进行了RT PCR半定量验证 ,结果与芯片一致 ,即D3和Y5基因在E组中的表达水平显著高于F组 [D3受体 :(0 .2 6± 0 .0 4 )vs.(0 .16± 0 .0 4 ) ;Y5受体 :(0 .94± 0 .10 )vs .(0 .6 8± 0 .0 9) ],而与正常对照组和阴性对照组间未见明显差异。结果表明 :小剂量红霉素可以改善糖尿病胃瘫大鼠的胃排空 ,其疗效有明显的个体差异 ;红霉素有效组多巴胺受体D3(DRD3)和神经肽受体Y5 (NPYY5 )表达水平显著高于红霉素无效组。提示这两种受体可能与红霉素促动力作用的易感性相关。
The gastric emptying in diabetic gastroparesis rats was determined by nuclide method and the model of diabetic gastroparesis was established. According to this model, we observed the effect of low dose erythromycin (3 mg kg) Empty effect. The results showed that the drug significantly reduced the one-hour residual rate of gastric emptying (R1H) (36.3% ± 6.8% vs. 36% ± 3.8%); however, this effect showed significant individual differences, According to the standard set into erythromycin effective group (E group) and invalid group (F group), found that there was a significant difference between the two groups. The receptor-related gene microarray was used to screen the differentially expressed genes in rat antral tissues. Results Ten differentially expressed genes were screened, and the semi-quantitative RT-PCR results of dopamine receptor D3 and neuropeptide Y receptor Y5 gene were obtained. The results were consistent with that of the chip, that is, the expression levels of D3 and Y5 genes in group E were significantly higher In group F [D3 receptor: (0.226 ± 0.04) vs. (0.16 ± 0.04); Y5 receptor: (0.94 ± 0.10) vs. (0. 6 8 ± 0. 0 9)], but no significant difference with the normal control group and negative control group. The results showed that low doses of erythromycin can improve gastric emptying in diabetic rats with gastroparesis, and the curative effect is obviously individual differences. Expression of dopamine receptor D3 (DRD3) and neuropeptide receptor Y5 (NPYY5) in erythromycin effective group Significantly higher than the level of erythromycin invalid group. Suggesting that these two receptors may be related to the susceptibility of erythromycin-induced motility.