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采用不脱钙骨切片技术 ,配合四环素双标记和骨组织形态计量及铝染色研究了注入不同浓度的铝及不同的肾功能对骨质形成和矿化作用的影响。实验大鼠分为 5组 :对照组 (C) ;低剂量铝组 (LDA) ;高剂量铝组 (HDA) ;慢性肾功能不全组(CRF) ;慢性肾功能不全加高剂量铝组 (CRF HDA)。实验结果表明 :可见HDA、CRF HDA组血清铝、骨铝含量明显增多。C、LDA组胫骨上段组织在荧光显微镜下骨小梁表面有清晰的四环素双标记线 ,该线粗细一致排列整齐 ,间距相等。CRF组的骨组织内双标记间距宽窄不一。HDA及CRF HDA组骨组织内四环素荧光显示的部位较少 ,少见双标线 ,多为单标线 ,粗细不一 ,融合增宽。在四环素标记线表面可见大量类骨质沉积 ,尤以CRF HDA组为甚 ,表明骨质形成及矿化严重受阻。骨铝染色显示HDA、CRF HDA组的骨及类骨质界面有粗细较一致的红色线条状物 ,提示铝染色阳性。实验研究结果说明铝尤其是高铝剂量可以抑制成骨细胞活性 ,铝可能起类骨质钙化的抑制剂作用。
The effects of different concentrations of aluminum and different renal functions on bone formation and mineralization were studied by using non-decalcified osteotomy technique combined with tetracycline double labeling and bone histomorphometry and aluminum staining. The experimental rats were divided into 5 groups: control group (C), low dose aluminum group (LDA), high dose aluminum group (HDA), chronic renal insufficiency group (CRF), chronic renal insufficiency plus high dose aluminum group HDA). Experimental results show that: visible HDA, CRF HDA serum aluminum, bone aluminum content increased significantly. C, the upper tibia tissue of LDA group under the fluorescent microscope trabecular bone surface with a clear double tetracycline double line, the thickness of the line uniform arrangement of neat, equal spacing. CRF group of double-spaced bone marker spacing varies. HDA and CRF HDA group of bone tissue tetracycline fluorescence showed less common, rare double-labeled, mostly monosyllabic, thickness varies, broadening. A large number of osteoid deposits were seen on the tetracycline surface, especially in the CRF HDA group, indicating that bone formation and mineralization were severely hampered. Bone-aluminum staining showed that red and blue lines with similar thickness were found on the bone and bone-like interface of HDA and CRF HDA groups, suggesting that aluminum staining was positive. Experimental results show that aluminum, especially high-aluminum dose can inhibit osteoblast activity, aluminum may play the role of osteocalcin inhibitor.