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利用组织学及组织化学的方法来观察不同时期低温灌注保存处理的12只狗的睾丸和18只人的睾丸结果表明:狗睾经上述处理后24h正常。人睾在48h仍呈现正常形态和结构,72h则出现部分生精上皮细胞变性,间质细胞SDH、附睾管ALPase下降。提示用低温灌注冷存的睾丸可以考虑作为睾丸移植的供睾。
The histological and histochemical methods were used to observe the testes of 12 dogs treated with hypothermic perfusion at different stages and the testes of 18 individuals showed that the dog’s testis was normal after 24 hours. The human testis still showed normal morphology and structure at 48 h, and at 72 h, some seminiferous epithelial cells degenerated, and the stromal cell SDH and epididymal duct ALPase decreased. It is suggested that hypothermic perfusion of cryopreserved testes can be considered as testicular transplantation for testis.