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一般认为抗肿瘤药物治疗的根本目的是要消灭有增殖能力的肿瘤干细胞。能形成克隆的细胞即为干细胞,而克隆形成实验是评价药物抗肿瘤作用的可靠指标。克隆形成实验是目前最受注意的抗肿瘤药物筛选方法之一。1 材 料 1细胞株 人肝癌细胞株QGY-7703及人肺癌细胞株SPC-A-1由吉
It is generally believed that the fundamental purpose of anti-tumor drug therapy is to eliminate cancer stem cells that have proliferative capacity. The cells that can form clones are stem cells, and the cloning experiment is a reliable indicator for evaluating the anti-tumor effect of drugs. Clone formation assays are one of the most popular methods for screening antitumor drugs. 1 Materials 1 Cell line Human hepatoma cell line QGY-7703 and human lung cancer cell line SPC-A-1