论文部分内容阅读
目的:建立通关藤中通关藤苷G和I的含量测定方法,并比较不同产地通关藤中两者的含量。方法:应用HPLC法,采用Ecosil C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(48∶52)为流动相,流速1.0 m L·min-1,检测波长230 nm,柱温30℃。结果:通关藤中通关藤苷G和I分别在0.412 4~4.124 0μg(r=0.999 5)和0.159 6~1.596 0μg(r=0.999 7)范围内线性关系良好,平均回收率分别为99.5%(RSD=2.4%)和100.0%(RSD=2.4%),不同产地药材中通关藤苷G和I含量范围分别为0.472%~0.882%和0.118%~0.362%。结论:本法准确度,重复性好,专属性强,可控制药材的质量;且不同产地药材中通关藤苷G和I 2种成分含量有一定差异性,其中云南丽江含量最高,可指导临床用药。
OBJECTIVE: To establish a method for the determination of ginsenosides G and I in Thunberg vine, and to compare the content of G and I in Thunberg vine. Methods: The mobile phase was acetonitrile-water (48:52) at a flow rate of 1.0 mL · min-1 using an Ecosil C18 column (4.6 mm × 150 mm, 5 μm) 30 ° C. Results: The linear correlation was found between the concentrations of gan and ganinol in the range of 0.412 4 ~ 4.124 0 μg (r = 0.999 5) and 0.159 6 ~ 1.596 0 μg (r = 0.999 7), respectively. The average recoveries were 99.5% RSD = 2.4%) and 100.0% (RSD = 2.4%). The contents of stannous glycosides G and I in different medicinal materials ranged from 0.472% to 0.882% and from 0.118% to 0.362%, respectively. Conclusion: The accuracy, repeatability and specificity of this method can be used to control the quality of medicinal materials. There are some differences in the contents of stannase G and I in different origin medicinal materials, among which Lijiang in Yunnan Province has the highest content and can guide clinical Medication.