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目的研究临床分离医院感染细菌耐消毒剂基因携带情况,为科学使用消毒剂提供依据。方法采用聚合酶链式反应(PCR)分析方法,对20株临床分离多重耐药细菌抗消毒剂基因进行检测。结果 20株多重耐药菌中,包括革兰阴性菌18株,革兰阳性菌2株。有7株菌检出抗消毒剂基因qacE△1,检出率为35%。结论医院临床分离的多重耐药菌,抗消毒剂基因qacE△1携带率为35%,提示在日常消毒工作中应关注抗消毒剂菌株的形成。
Objective To study the clinical isolates of nosocomial bacterial resistance to disinfectants in hospital and to provide basis for the scientific use of disinfectants. Methods Polymerase chain reaction (PCR) method was used to detect the antimicrobial agents in 20 clinically isolated and multi-drug resistant bacteria. Results Among the 20 multidrug-resistant bacteria, 18 were Gram-negative bacteria and 2 were Gram-positive bacteria. Seven strains of bacteria detected anti-disinfectant gene qacE △ 1, the detection rate was 35%. Conclusions The qacE △ 1 carrier rate of multidrug-resistant and anti-disinfectant genes isolated clinically in hospitals is 35%, suggesting that the formation of anti-disinfectant strains should be concerned in routine disinfection.