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目的 用体外培养法研究中药提取物 990 1对肿瘤HeLa细胞生长的抑制作用和作用机制。方法 采用MTT法和激光扫描共聚焦显微镜进行研究。结果 HeLa细胞在 990 1处理 3天后 ,测得该药的IC50 为 5 .12 μg/ml。用不同浓度的 990 1处理HeLa细胞 48h后 ,用PI染色 ,HeLa细胞核有形态变化 ,DNA含量明显减少 ,经统计学处理 ,与对照组间有显著性差异。结论 中药提取物 990 1可有效抑制HeLa细胞的生长 ,通过影响细胞的DNA而发挥作用。
Objective To study the inhibitory effect of Chinese herbal extract 990 1 on the growth of tumor HeLa cells and the mechanism of action. Methods The MTT method and laser scanning confocal microscope were used for the study. Results After 3 days of treatment with 990 1, HeLa cells had an IC50 of 5.12 μg/ml. HeLa cells were treated with different concentrations of 990 1 for 48 h. After PI staining, there were morphological changes in HeLa cell nucleus and DNA content was significantly reduced. Statistical analysis showed that there was a significant difference between the control group and HeLa cells. Conclusion Chinese herbal extract 990 1 can effectively inhibit the growth of HeLa cells and play a role in affecting the DNA of cells.