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亚铁毒是潜育性水稻土中限制水稻产量的主要因子。利用龙杂8503 /IR64的F2和等价的F3群体,在营养液中培养来定位耐亚铁毒的QTLs。通过构建101SSR标记的遗传连锁图谱来确定耐亚铁毒QTLs的位置和特性。借助叶片棕色斑点指数、株高和最大根长3个性状,利用营养液在水稻苗期来评价F2单株、F3群体和亲本龙杂8503、IR64,共检测到叶片棕色斑点指数、株高和最大根长的QTLs 20个,分布在水稻的10条染色体上,表明这些性状受多基因控制。控制叶片棕色斑点指数的QTLs分别定位在第1染色体的RM315 ~RM212、第2染色体的RM6 ~RM240和第4染色体的RM252 ~RM451之间。与前人的研究结果比较发现:1)位于第4染色体RM252~RM451之间的控制叶片棕色斑点指数的QTL与水稻功能图谱上控制叶绿素含量减少的QTL的位置一致。另一个位于第1染色体的RM315-RM212之间的控制叶片棕色斑点指数的QTL与水稻功能图谱上位于C178-R2635之间控制叶绿素含量的QTL连锁。2)位于第2染色体RM6-RM240之间的第3个控制叶片棕色斑点指数的QTL与位于RZ58-CDO686的控制钾吸收的QTL连锁。
Ferrous iron is a major limiting factor on paddy yield in gneid paddy soils. The F2 population of Longza 8503 / IR64 and the equivalent F3 population were used to locate the QTLs resistant to ferrous toxicity in nutrient solution. The location and identity of QTLs resistant to ferrous iron were determined by constructing the genetic linkage map of 101SSR markers. The leaf brown spot index, plant height and maximum root length were used to evaluate the leaf spot index, plant height and plant height of F2 individuals, F3 population and their parents Longzhe 8503 and IR64 by rice seedling stage. Twenty QTLs with the largest root length were distributed on 10 chromosomes of rice, indicating that these traits are controlled by multiple genes. QTLs controlling the brown spot index of leaves were located between RM315 and RM212 on chromosome 1, RM6 and RM240 on chromosome 2, and RM252 and RM451 on chromosome 4, respectively. Compared with the results of previous studies, 1) the QTL controlling the brown spot index of leaves between RM252 and RM451 on chromosome 4 is consistent with the QTL controlling the reduction of chlorophyll content in rice functional map. Another QTL controlling the leaf brown spot index between RM315-RM212 on chromosome 1 was linked to a QTL controlling chlorophyll content on rice functional map C178-R2635. 2) The third QTL controlling leaf brown spot index located between RM6 and RM240 on chromosome 2 was linked to the QTL controlling potassium uptake at RZ58-CDO686.