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改进环孢素A（CsA）的高效液相色谱法（HPLC）测定方法。方法：全血经乙醚提取后，吹干，用甲醇、稀HCI定容，正己烷脱脂后进样。流动相为乙腈：水（100：20），色谱法为YWG-C184.6×150mm，检测波长为214nm。结果：CsA与CsD峰分离效果良好，最低检测限为5ng（信噪比是2.65），最低检测浓度为10μg·L-1。提取步骤少，方法简便、快捷。结论：本方法适用于临床器官移植中CsA的大量快速的样本测定。
High Performance Liquid Chromatography (HPLC) Method for Improving Cyclosporin A (CsA). Methods: After the whole blood was extracted by ether, it was blown to dryness. After diluting with methanol and dilute HCI, the sample was degreased with n-hexane. The mobile phase was acetonitrile: water (100: 20). The chromatographic method was YWG-C18 4.6 × 150 mm and the detection wavelength was 214 nm. Results: CsA and CsD peaks were separated well with the lowest detection limit of 5 ng (signal to noise ratio of 2.65) and the lowest detection concentration of 10 μg · L -1. Less extraction steps, the method is simple and fast. Conclusion: This method is suitable for a large number of rapid sample determinations of CsA in clinical organ transplantation.