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目的:探讨明党参中苯丙素类化合物在不同器官中累积分布规律。方法:采用HPLC法对明党参植株不同器官中对甲氧基肉桂酸、伞形花内酯的含量进行测定。结果:明党参根、茎叶、花葶、花及果实等器官中均含有对甲氧基肉桂酸、伞形花内酯。对甲氧基肉桂酸含量以根中为高,伞形花内酯含量以花中最高。结论:建立了呋喃香豆素生物合成前体成分对甲氧基肉桂酸和伞形花内酯的HPLC含量测定方法,为进一步开展明党参中香豆素化合物的生物合成研究提供了基础。
Objective: To investigate the accumulation and distribution of phenylpropanoids in different organs of Codonopsis Pilosula. Methods: The content of p-methoxycinnamic acid and umbellate in different organs of Codonopsis Pilosula were determined by HPLC. Results: Codonopsis root, stem and leaf, Scape, flowers and fruits and other organs contain p-methoxycinnamic acid, umbellate. The content of p-methoxycinnamic acid was high in root, and the content of umbelliferone was the highest in flower. CONCLUSION: The HPLC method for the determination of methoxycinnamic acid and ursovalenone, the precursor of furanocoumarin biosynthesis, has been established, which provides the basis for further research on the biosynthesis of coumarin compounds in Radix Codonopsis.