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目的:通过监测哮喘儿童血清白细胞介素(IL)-25、嗜酸细胞趋化因子(Eotaxin)表达水平,探讨其水平变化及其在哮喘发病中的作用。方法:收集哮喘急性发作期、缓解期儿童及健康儿童的静脉血标本,用双抗体夹心ELISA法测定血清IL-25、Eotaxin的水平。结果:哮喘儿童急性发作期组血清IL-25水平(56.75±11.68)ng/L显著高于缓解期组(47.09±10.96)ng/L及健康对照组(45.77±10.43)ng/L,差异有统计学意义(P<0.05),缓解期组与健康对照组比较差异无统计学意义;哮喘急性发作期组儿童血清Eotaxin水平(120.95±23.97)ng/L显著高于缓解期组(105.47±22.01)ng/L及健康对照组(105.01±18.47)ng/L,差异有统计学意义(P<0.05),缓解期组与健康对照组比较差异无统计学意义。哮喘儿童血清IL-25水平与Eotaxin之间存在明显正相关关系(r=0.642,P<0.01)。结论:哮喘儿童血清IL-25及Eotaxin水平能反应哮喘气道炎症活动情况及疾病严重程度,两者存在正相关关系。
Objective: To investigate the level of serum interleukin (IL) -25 and eotaxin in asthmatic children and its role in the pathogenesis of asthma. Methods: Venous blood samples of children and healthy children during the acute attack and remission period were collected. The levels of serum IL-25 and Eotaxin were measured by double antibody sandwich ELISA. Results: The level of IL-25 in acute exacerbation group (56.75 ± 11.68) ng / L was significantly higher than that in remission group (47.09 ± 10.96) ng / L and healthy control group (45.77 ± 10.43) ng / L (P <0.05). There was no significant difference between the remission group and the healthy control group. Serum Eotaxin level was 120.95 ± 23.97 ng / L in the asthma exacerbation group was significantly higher than that in the remission group (105.47 ± 22.01 (ng / L) and healthy control group (105.01 ± 18.47) ng / L, the difference was statistically significant (P <0.05). There was no significant difference between remission group and healthy control group. There was a significant positive correlation between serum IL-25 level and Eotaxin in asthmatic children (r = 0.642, P <0.01). Conclusion: The levels of serum IL-25 and Eotaxin in asthmatic children can reflect the airway inflammation activity and the severity of asthma. There is a positive correlation between them.