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目的:观察瓜蒌薤白汤对大鼠肺纤维化形成阶段(造模后14～28 d)肺部病理损害和肺组织去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响。方法:普通级雌性Wistar大鼠随机分为正常组、模型组、泼尼松组、瓜蒌薤白汤组。除正常组外,其余各组均采用气管内一次性注入平阳霉素(0.003 g·kg-1体重)复制肺纤维化模型,造模14 d后,泼尼松组以泼尼松0.004 g·kg-1体重ig,瓜蒌薤白汤组以全瓜蒌2.04 g·kg-1体重,薤白1.02 g·kg-1体重ig,第28天处死动物,比较各组肺指数、肺纤维化程度、肺组织DA,NE,5-HT含量。结果:模型组肺纤维化程度重于正常组、泼尼松组与瓜蒌薤白汤组(P<0.05),肺指数高于正常组与各用药组(P<0.05),肺组织DA,NE,5-HT含量高于正常组、泼尼松组(P<0.05);DA:模型组(60.57±8.47)ng·g-1,正常组(20.59±6.68)ng·g-1,泼尼松组(18.44±4.16)ng·g-1;NE:模型组(37.01±4.99)ng·g-1,正常组(26.91±1.90)ng·g-1,泼尼松组(23.06±5.64)ng·g-1;5-HT:模型组(88.87±5.02)ng·g-1,正常组(20.31±4.24)ng·g-1,泼尼松组(69.18±9.00)ng·g-1;瓜蒌薤白汤组NE含量(46.31±4.55)ng·g-1明显高于模型组,DA(27.48±3.20)ng·g-1,5-HT(48.90±8.47)ng·g-1含量低于模型组(P<0.05)。结论:瓜蒌薤白汤具有减轻肺纤维化模型形成阶段肺病理改变的作用;肺纤维化形成阶段肺中DA,NE,5-HT升高;瓜蒌薤白汤能抑制模型肺DA,5-HT含量的升高,增加模型肺中NE含量。
Objective: To observe the pathological changes of pulmonary pathology and the changes of norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5- HT) content of the impact. Methods: The normal female Wistar rats were randomly divided into normal group, model group, prednisone group and melon soup group. In addition to the normal group, pulmonary fibrosis was induced by intratracheal instillation of Pingyangmycin (0.003 g · kg-1 body weight) once a day, and prednisone was given as 0.004 g · kg-1 body weight ig, melon 蒌 薤 Baitang group with whole melon 蒌 2.04 g · kg-1 body weight, 薤 white 1.02 g · kg-1 body weight ig, the animals were sacrificed on the 28th day, the lung index, lung fibrosis, Lung tissue DA, NE, 5-HT content. Results: The degree of pulmonary fibrosis in model group was heavier than that in normal group, prednisone group and Gua Xie Baitang group (P <0.05), and the lung index was higher than that in normal group and each drug group (P <0.05) (P <0.05); DA: model group (60.57 ± 8.47) ng · g-1, normal group (20.59 ± 6.68) ng · g-1, prednisone group (18.44 ± 4.16) ng · g-1 in the untreated group, NE (37.01 ± 4.99) ng · g-1 in the model group, 26.91 ± 1.90 ng · g-1 in the normal group, 23.06 ± 5.64 in the prednisone group, ng · g-1; 5-HT: (88.87 ± 5.02) ng · g-1 in model group, 20.31 ± 4.24 ng · g-1 in normal group and 69.18 ± 9.00 ng · g-1 in prednisone group (46.31 ± 4.55) ng · g-1 in Guizhuhuibutang group was significantly higher than that in model group (27.48 ± 3.20) ng · g-1,5-HT (48.90 ± 8.47) ng · g-1 Lower than model group (P <0.05). CONCLUSION: Guadangbaitang can alleviate the pathological changes of lung during the formation of pulmonary fibrosis model. The levels of DA, NE and 5-HT in the lung during the formation of pulmonary fibrosis are increased. The content of NE increased in model lungs.