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目的观察急性脑缺血时肾脏微循环超微结构的改变，从而探讨脑缺血时其它脏器微循环的相应性变化，为治疗建立机体的整体观提供依据。方法选用健康ＳＤ大鼠，戊巴比妥钠麻醉后、结扎颈总动脉，建立急性脑缺血模型。动物随机分为三组：假手术组、盐水对照组、药物治疗组。按实验要求观察后处死动物，立即取材，用常规超薄切片技术制样，作电镜观察。结果急性脑缺血时肾小球毛细血管有缺血性改变。毛细血管内皮细胞体积缩小，电子密度深，核固缩，染色质分布异常，核膜不清晰。胞浆减少，细胞器稀疏且数量少。毛细血管基底膜浑浊，电子密度减低。基底膜外足细胞形态不规则，体积缩小，足细胞胞浆内空泡较多，细胞器减少，足突排列紊乱。结论急性脑缺血后，产生的微循环障碍不仅限于脑微循环。脑缺血可以引起全身微循环紊乱，可以导致肾小球毛细血管微循环失调。提示机体微循环是个统一的、有密切联系的网络系统。临床治疗时应建立整体现。
Objective To observe the ultrastructural changes of renal microcirculation during acute cerebral ischemia, so as to explore the corresponding changes of microcirculation in other organs during cerebral ischemia, and provide evidence for the establishment of whole body view. Methods Healthy SD rats were anesthetized with pentobarbital sodium and the common carotid arteries were ligated to establish acute cerebral ischemia model. Animals were randomly divided into three groups: sham operation group, saline control group, drug treatment group. Animals were sacrificed according to the requirements of the experiment, immediately drawn, and the samples were prepared by conventional ultra-thin sectioning technique for electron microscopy. Results When acute cerebral ischemia, glomerular capillaries have ischemic changes. Capillary endothelial cells shrink in volume, deep electron density, nuclear pyknosis, abnormal distribution of chromatin, the nuclear membrane is not clear. Less cytoplasm, sparse and fewer organelles. Capillary basement membrane cloudy, electron density decreased. Podocytes outside the basement membrane irregular shape, size, podocytes within the cytoplasm more vacuoles, organelles decreased foot disorder arranged. Conclusion Acute cerebral ischemia, the resulting microcirculation is not limited to cerebral microcirculation. Cerebral ischemia can cause systemic microcirculation disorders, can lead to glomerular capillary microcirculation disorders. Tip body microcirculation is a unified, closely linked network system. Clinical treatment should be established as a whole now.