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以合丰35、黑农44和吉林35的胚尖为外植体,分别考察了不同浓度的卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响。结果表明,不定芽诱导率合丰35和吉林35在卡那霉素100 mg.L-1时与对照差异不显著,黑农44在100 mg.L-1处理时显著低于对照;芽数吉林35在卡那霉素100 mg.L-1时显著低于对照并高于其他浓度,而合丰35与黑农44在100 mg.L-1时芽数与对照差异不显著;3种基因型大豆胚尖不定芽伸长均在卡那霉素浓度为100 mg.L-1时受到显著抑制。草铵膦浓度在0.2～1.2 mg.L-1时对合丰35和吉林35的芽数没有影响,但黑农44在1.0 mg.L-1时芽数开始显著低于对照;芽长合丰35、黑农44和吉林35分别在0.6、0.2和0.2 mg.L-1时显著低于对照。因此,合丰35、黑农44和吉林35的适宜卡那霉素的筛选浓度为100 mg.L-1,草铵膦浓度分别为0.6、0.2和0.2mg.L-1。
The effects of different concentrations of kanamycin and glufosinate on the induction of adventitious buds at the embryogenesis of different genotypes were studied by using the embryo tips of Hefeng 35, Heinong 44 and Jilin 35 as explants. The results showed that the induction rate of adventitious buds of Hefeng 35 and Jilin 35 was not significantly different from that of the control at the concentration of 100 mg.L-1 of kanamycin. The treatment with 100 mg.L-1 of Heinong 44 was significantly lower than that of the control Jilin 35 was significantly lower than kanamycin at the concentration of 100 mg.L-1 and higher than the other treatments, while the difference between bud number and Hefei 35 and Heinong 44 at 100 mg.L-1 was not significant. Genomic soybean embryo adventitious bud elongation at kanamycin concentration of 100 mg.L-1 was significantly inhibited. Glufosinate concentrations of 0.2-1.2 mg.L-1 had no effect on the shoot number of Hefeng 35 and Jilin 35, but the shoot number of Heinong 44 at 1.0 mg.L-1 was significantly lower than that of the control; Feng 35, Heinong 44 and Jilin 35 were significantly lower than the control at 0.6, 0.2 and 0.2 mg.L-1, respectively. Therefore, the screening kanamycin levels of Hefeng 35, Heinong 44 and Jilin 35 were 100 mg.L-1, and the concentrations of glufosinate were 0.6, 0.2 and 0.2 mg.L-1, respectively.