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目的:研究枯草芽孢杆菌拮抗幽门螺杆菌的能力及分离纯化其抑菌活性物质。方法:以幽门螺杆菌国际标准株NCTC11637为指示菌,采用打孔法检测枯草芽孢杆菌抑制幽门螺旋杆菌能力;挑选拮抗能力最强菌株,对其活性物质进行分离纯化;质谱检测活性物质分子量。结果:枯草芽孢杆菌发酵上清液具有显著抑制幽门螺旋杆菌的能力,其中枯草芽孢杆菌KM抑制幽门螺旋杆菌的能力最强;活性物质经分离纯化后,质谱检测分子量为3402.524D,推测为Subtilosin A结构类似物;10株枯草芽孢杆菌Subtilosin A的前体基因的表达水平与抗幽门螺旋杆菌的能力呈正相关。结论:枯草芽孢杆菌具有不同程度体外拮抗幽门螺旋杆菌能力,其抑菌机制与分泌抗菌肽Subtilosin A结构类似物相关,该类物质可作为治疗幽门螺杆菌感染的候选药物。
Objective: To study the ability of Bacillus subtilis to antagonize Helicobacter pylori and to isolate and purify its antibacterial substance. Methods: The Helicobacter pylori international standard strain NCTC11637 was used as the indicator to detect the ability of Bacillus subtilis to inhibit Helicobacter pylori by the punching method. The strongest antagonistic strain was selected to separate and purify the active substance. The molecular weight of the active substance was detected by mass spectrometry. Results: The Bacillus subtilis fermentation supernatant had the ability to inhibit H. pylori significantly. Among them, Bacillus subtilis KM had the strongest inhibitory effect on Helicobacter pylori. The purity of the active substance after purification was 3402.524D, which was estimated to be Subtilosin A Structural analogs. The expression level of 10 precursor genes of Bacillus subtilis Subtilosin A was positively correlated with the ability of anti-Helicobacter pylori. CONCLUSION: Bacillus subtilis has different degrees of in vitro antagonism against Helicobacter pylori, and its antibacterial mechanism is related to the secretion of the structural analog of Subtilosin A, which can be used as a candidate drug for the treatment of Helicobacter pylori infection.