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于1997年9月在青岛红岛养殖场采集中国对虾,给对虾腹腔注射不同剂量的海藻多糖和北虫草多糖后,用生化法测定其血清中的溶菌酶(LSZ)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)和过氧化物酶(POD)活性的动力学变化。结果表明,注射1.0%北虫草多糖及1.0%海藻多糖48h后,对虾血清中的LSZ活性由对照组的0.25U/ml分别增至0.88U/ml和0.75U/ml,POD活性由对照组的0.11U/mg分别增至2.2U/mg和1.96U/mg;注射72h后,ALP活性由对照组的1.2U/mg分别增至48U/mg和25U/mg,ACP活性由对照组的4.5U/mg增至30U/mg和17U/mg,而血清蛋白的变化较为稳定。LSZ、ALP和ACP的活性变化最为显著,说明海藻多糖和北虫草多糖作为免疫药物能够增强中国对虾的免疫功能。
In September 1997, Chinese shrimp were collected from Qingdao Red Island farm and different doses of seaweed polysaccharide and Cordyceps polysaccharide were injected intraperitoneally to the shrimp. The levels of lysozyme (LSZ), alkaline phosphatase (ALP) ), Acid phosphatase (ACP) and peroxidase (POD) activity. The results showed that the activity of LSZ in shrimp serum increased from 0.25U / ml to 0.88U / ml and 0.75U / ml respectively after injection of 1.0% Cordyceps polysaccharide and 1.0% seaweed polysaccharide for 48h. The activity of POD from control group 0.11U / mg to 2.2U / mg and 1.96U / mg respectively; After 72h injection, ALP activity increased from 1.2U / mg to 48U / mg and 25U / mg respectively in the control group. ACP activity increased from 4.5U / Mg increased to 30U / mg and 17U / mg, while serum protein changes more stable. The changes of activity of LSZ, ALP and ACP were the most significant, indicating that seaweed polysaccharide and Cordyceps polysaccharide as immune drugs can enhance the immune function of shrimp in China.