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[目的]研究异硫氰酸苯乙酯(PEITC)体外实验中对人胃癌BGC-823细胞的增殖抑制作用及诱导其凋亡相关分子机制。[方法]应用MTT法、Annexin V/PI双染法检测PEITC人胃癌BGC-823细胞增值以及凋亡的影响;应用RT-PCR以及Western Bolt检测PEITC诱导胃癌细胞凋亡相关基因蛋白的表达情况。[结果]PEITC作用人胃癌细胞BGC-823给药24h后,与空白组比较,能显著抑制胃癌细胞的增殖,抑制作用与浓度和时间成依赖关系,诱导胃癌细胞的凋亡,能上调Bax表达,下调Bcl-2、Bcl-xl、Survivin等基因表达,激活Caspase-3、Caspase-9、PARP活性。[结论]PEITC能有效抑制人胃癌BGC-823细胞的增殖,其机制可能是通过激活Caspase家族蛋白酶活性而诱导胃癌细胞凋亡。
[Objective] To investigate the inhibitory effect of phenylethyl isothiocyanate (PEITC) on the proliferation of human gastric cancer cell line BGC-823 in vitro and the related molecular mechanism of its apoptosis. [Methods] The proliferation and apoptosis of PEITC human gastric cancer cell line BGC-823 were detected by MTT assay and Annexin V / PI double staining method. The expression of PEITC-induced apoptosis-related gene protein was detected by RT-PCR and Western Bolt. [Result] After treated with PEITC for 24 h, the gastric cancer cell BGC-823 could significantly inhibit the proliferation of gastric cancer cells in a dose-dependent and time-dependent manner and induce the apoptosis of gastric cancer cells and up-regulate the expression of Bax , Downregulate the gene expression of Bcl-2, Bcl-xl, Survivin and activate Caspase-3, Caspase-9, PARP activity. [Conclusion] PEITC can effectively inhibit the proliferation of human gastric cancer cell line BGC-823 by inducing caspase family protease activity and inducing gastric cancer cell apoptosis.