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昆虫可以利用小干扰RNA(siRNA)机制干扰体内RNA病毒的增殖,从而获得对该病毒的免疫能力。通过第二代测序技术对蚊子的小RNA进行高通量测序,再通过生物信息学方法寻找其中的RNA病毒序列,发现在我国云南地区的白纹伊蚊和致倦库蚊体内存在Marayo,Omsk hemorrhagic fever和Ilheus等RNA病毒的基因片段。至此建立了发现媒介昆虫体内携带病毒的一种新方法,可用于媒介昆虫携带RNA病毒的本底调查。
Insects can interfere with the proliferation of RNA viruses in vivo using small interfering RNA (siRNA) machinery to gain immunity to the virus. High-throughput sequencing of mosquitoes’ small RNAs by second-generation sequencing technology and sequencing of the RNA viruses by bioinformatics methods revealed the presence of Marayo, Omsk in Aedes albopictus and Culex pipiens quinquefasciatus in Yunnan, China. hemorrhagic fever and Ilheus and other RNA virus gene fragments. So far, a new method of detecting virus carried by vector insects has been established, which can be used for background investigation of vector-borne RNA viruses.