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目的:观察中药方剂大青叶、马齿苋、甘草组合物在流感病毒H1N1感染的MDCK细胞模型中抗流感病毒的作用。方法:建立流感病毒H1N1感染MDCK细胞模型,试验分为药物组、正常对照组和病毒对照组,37℃、5%CO2培养箱中培养18h后观察各组细胞的增殖变化,收集细胞上清液,检测各组流感病毒血凝效价及ELISA法检测TNF-ɑ、IFN-γ、IL-2与IL-6的含量。结果:与病毒对照组相比,用药后流感病毒的血凝效价从2-8降为2-4,二组流感病毒血凝效价比较,P<0.01。与病毒对照组相比,中药方剂大青叶、马齿苋、甘草组合物组TNF-ɑ水平下降(P<0.05),IFN-γ水平升高(P<0.05),差异均有统计学意义,IL-2有升高的趋势(P>0.05)。结论:用流感病毒H1N1感染MDCK细胞模型,可以较好的评价中药抗流感病毒的作用。中药方剂大青叶、马齿苋、甘草组合物可通过降低TNF-ɑ的含量以减轻病毒对宿主细胞的免疫病理损伤,并通过促进IFN-γ、IL-2等的分泌以提高机体的抗病毒能力;同时该中药组合物可较好的抑制流感病毒的复制。
OBJECTIVE: To observe the anti-influenza virus effect of the traditional Chinese medicine prescription of Folium Isatidis, Portulaca oleracea and Glycyrrhiza composition in the MDCK cell model infected by influenza virus H1N1. Methods: The MDCK cell model of influenza virus H1N1 infection was established. The experiment was divided into drug group, normal control group and virus control group. The proliferation of each group was observed after cultured in 37 ℃, 5% CO2 incubator for 18 hours. . The hemagglutination titer of influenza virus in each group and the content of TNF-ɑ, IFN-γ, IL-2 and IL-6 were detected by ELISA. Results: Compared with the control group, the hemagglutination titer of the influenza virus was decreased from 2-8 to 2-4 after treatment, and the titer of the blood coagulation of the two groups was P <0.01. Compared with the virus control group, the levels of TNF-ɑ and the level of IFN-γin the prescriptions of Folium Isatidis, Portulaca oleracea and Glycyrrhizin decreased (P <0.05), the differences were statistically significant , IL-2 has an increasing trend (P> 0.05). Conclusion: The MDCK cell model infected with influenza virus H1N1 can better evaluate the anti-influenza virus effect of traditional Chinese medicine. Traditional Chinese medicine Folium, purslane, licorice composition by reducing the content of TNF-减 to reduce the virus on the host cell immune pathological damage, and by promoting the secretion of IFN-γ, IL-2, etc. to improve the body’s resistance Virus ability; at the same time, the Chinese medicine composition can better inhibit the replication of influenza virus.