通过流式细胞术(FACS),利用细胞表面糖蛋白(Jlld.2)单克隆抗体研究了J l ld在新制备的小鼠脾脏B细胞上的表达,特别是细菌脂多糖(lipopolysaccharide,LPS)刺激后Jl ld表达的变化。发现浮力密度较高的小B细胞高度表达Jl ld(Jlld~高),而Jl ld低细胞则为浮力密度较低的大B细胞。用LPS刺激脾脏高浮力密度小B细胞,可诱导Jl ld表达的降低。这些结果表明,B细胞活化后Jl ld的表达降低,Jl ld的高和低并不是一个稳定的细胞族系(lineage)标志。 The expression of Jlld on newly prepared mouse splenic B cells was studied by flow cytometry (FACS) using the monoclonal antibody against cell surface glycoprotein (Jlld.2), in particular, lipopolysaccharide (LPS) Jl ld after stimulation of expression changes. Small B cells with higher buoyant density were found to be highly expressed (Jlld - J), whereas Jlld low cells were large B cells with low buoyancy. LPS stimulation of spleen high buoyancy density of small B cells, Jl ld induced expression can be reduced. These results indicate that Jlld expression is decreased after B cell activation and that high and low Jlld is not a stable lineage marker.