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培育多抗性基因聚合系可为抗性育种提供重要的材料基础。通过抗源与不同恢复系和保持系进行杂交、多代回交和标记辅助选择,聚合优良的抗稻白叶枯病和稻褐飞虱基因研究,分别获得了基于不同恢复系和保持系遗传背景的抗稻白叶枯病双基因Xa21Xa23聚合系4个,这些抗性聚合系对白叶枯病的表现为高抗;分别获得抗稻白叶枯病和稻褐飞虱的抗性基因Xa21Xa23Bph24(t)聚合系2个和Xa23 Bph24(t)聚合系4个,双抗性聚合系对稻白叶枯病达到高抗水平,对稻褐飞虱的抗性接近高抗水平。结果表明,即使在Xa23表达了高水平抗性的情况下,聚合双基因Xa21Xa23仍可以在一定程度上提高聚合系对稻白叶枯病的抗性水平;此外,聚合系的两类抗性基因的抗性表达相互之间没有影响。抗性基因聚合系的培育提高了恢复系和保持系的育种价值,同时也为进一步聚合更多的重要抗性基因、培育多抗性水稻品种打下了坚实基础。
Breeding multiresistance gene Polymerisation can provide an important material basis for resistance breeding. Through the study of resistance to different restorer lines and maintainer lines, multiple generations of backcross and marker-assisted selection, excellent resistance to rice bacterial leaf blight and rice brown planthopper, we obtained the genetic backgrounds based on different restorer and maintainer lines The results showed that the resistant lines Xa21Xa23 were resistant to bacterial leaf blight and resistant to rice leaf blight. The resistance genes Xa21Xa23Bph24 (t) were resistant to bacterial leaf blight and brown planthopper, respectively 2 and Xa23 Bph24 (t). The bi-resistant polymeric lines reached high level of resistance to rice bacterial leaf blight and the resistance to the brown planthopper was near high level. The results showed that even though Xa23 expressed high level of resistance, the polymeric double gene Xa21Xa23 could still improve the resistance of the polymer lines to rice bacterial leaf blight to some extent. In addition, two types of resistance genes The resistant expression has no effect on each other. The breeding of resistance gene polymerization system enhanced the breeding value of restorer lines and maintainer lines, and laid a solid foundation for the further polymerization of more important resistance genes and breeding of multi-resistant rice varieties.