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我们在本所建立了小型简易的裸鼠饲养与实验室,利用国产超净空气层流架饲养BALB/C系裸小鼠获得成功。裸鼠生长情况良好,正常裸鼠的寿命可存活10个月以上,荷瘤裸鼠的寿命最长者能达到286天。经病理组织学检查,裸鼠主要脏器的组织结构和细胞形态正常,未发现寄生虫或细菌等微生物的感染及其炎症病变。而且,我们应用BALB/C系裸小鼠建立了2个人胃癌移植瘤株,为开展人类胃癌的实验治疗提供了理想的动物模型。作者认为,应用层流架并在裸鼠笼上辅以空气过滤帽对于饲养SPF(无特定病原菌)实验动物是可行的,针对国情具有推广价值。
We established a small and simple nude mice breeding and laboratory in the Institute, and successfully raised BALB/C nude mice using domestic ultra-clean air laminar flow. The growth of nude mice was good, and the lifespan of normal nude mice survived for more than 10 months. The longest lifespan of tumor-bearing nude mice was 286 days. Histopathological examination showed that the main organs of the nude mice had normal histological structure and cell morphology, and no infections such as parasites or bacteria and inflammatory lesions were found. Furthermore, we established two human gastric cancer xenografts in BALB/C nude mice and provided an ideal animal model for the experimental treatment of human gastric cancer. The author believes that applying a laminar flow frame and supplementing an air filter cap on a nude cage is feasible for feeding experimental animals with SPF (no specific pathogenic bacteria), and has an extension value for national conditions.