目的:建立HPLC法测定人参鹿茸丸中补骨脂、异补骨脂素含量的方法。方法:采用大连依利特HypersilODS-C18色谱柱,以甲醇-水(55∶45)为流动相,检测波长:246 nm,流速:1.0 mL/min。结果:补骨脂素在0.026 3～0.316 1μgr,=0.999 9,平均加样回收率98.32%;异补骨脂素在0.02908～0.3489μgr,=0.9999,平均加样回收率97.74%;范围内线性关系良好。结论:本方法操作简便、方法可靠、结果准确、灵敏度高、重现性好、可用于该制剂的质量控制。 Objective: To establish a HPLC method for the determination of psoralen and isopsoralen in ginseng pilose antler pill. Methods: Hypersil ODS-C18 column was used in this study. The mobile phase was methanol-water (55:45). The detection wavelength was 246 nm and the flow rate was 1.0 mL / min. Results: The average recoveries of psoralen in the range of 0.026 3-0.316 1 μg r = 0.999 9 were 98.32%. The average recovery of isopsoralen was 0.02908-0.3489 μg r = 0.9999 with the average recovery of 97.74% Good relationship. Conclusion: The method is simple, reliable, accurate, sensitive and reproducible. It can be used for the quality control of this preparation.