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背景:神经生长因子(nervegrowthfactor,NGF)在神经元的生长、存活、分化、保护神经元的退行性改变及神经损伤的自我保护与修复中具有重要作用,目前NGF保护神经元的分子病理学机制还有许多需要深入探讨的。目的:观察脑缺血老年大鼠部分脑区及小脑内源性神经生长因子的变化。设计:完全随机对照实验研究。地点与材料:本研究的地点为广州军区广州总医院实验科。材料为36只SD大鼠2～2.5年龄,雌雄不限。干预:用夹闭两侧颈总动脉法建立不完全性脑缺血动物模型,将SD大鼠随机分成正常对照组(A组)、假手术组(B组)、缺血30min再灌注6h(C组)、12h(D组)、24h(E组)、48h(F组)、7d(G组)和14d(H组),用免疫组织化学ABC染色法检测部分脑区神经元及小脑NGF的表达。在透射电镜下观察各组神经元超微结构变化。主要观察指标:各组神经元NGF表达定性及定量观察。结果:除A、B组外,各组顶叶皮质神经元均有NGF表达,其中E组和G组中量表达,神经元数量分别为(58.4±9.18)mm2和(56.2±10.87)mm2;除A组、B组、F组和H组外各组海马均有少量表达,神经元数量C组为(28.8±6.42)mm2、D组(30.4±12.12)mm2、E组(24.2±5.18)mm2、G组(15.6±4.39)mm2;小脑均没有表达;再灌注超过48h组海马神经元和小脑蒲肯野细胞损伤较重,顶叶皮质神经元轻度水肿。
BACKGROUND: Nerve growth factor (NGF) plays an important role in the growth, survival and differentiation of neurons, the degenerative changes of protective neurons and the self-protection and repair of nerve injury. At present, the molecular pathological mechanism of NGF neurons protection There are many more needs to be explored. Objective: To observe the changes of endogenous nerve growth factor in some brain regions and cerebellum of aged rats with cerebral ischemia. Design: Complete randomized controlled experimental study. Venue and Materials: This study was conducted in the laboratory of Guangzhou General Hospital of Guangzhou Military Region. Materials for 36 SD rats 2 ~ 2.5 years of age, male or female. Intervention: The animal model of incomplete cerebral ischemia was established by occluding the common carotid artery on both sides. The SD rats were randomly divided into normal control group (A group), sham operation group (B group), reperfusion for 6 hours (Group C), 12h (group D), 24h (group E), 48h (group F), 7d (group G) and 14d (group H). Immunohistochemical ABC staining was used to detect the expression of NGF expression. The ultrastructural changes of neurons in each group were observed under transmission electron microscope. MAIN OUTCOME MEASURES: The qualitative and quantitative observation of NGF expression in neurons of each group. RESULTS: Except for groups A and B, NGF expression was found in the parietal cortex neurons in each group, and the number of neurons in the groups E and G was (58.4 ± 9.18) mm2 and (56.2 ± 10.87) mm2, respectively. The number of neurons in group C was (28.8 ± 6.42) mm2, group D was (30.4 ± 12.12) mm2, group E was (24.2 ± 5.18) mm in group A, group B, group F and group H respectively mm2 in group G and (15.6 ± 4.39) mm2 in group G, but no cerebellum was observed. In hippocampal neurons and cerebellum Purkinje cells, the neurons were more damaged and the parietal cortex neurons were slightly edematous.