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目的:研究不同强度的体外高压电场对肺癌细胞系A549细胞的黏附、迁移及侵袭等能力的影响。方法:选择处于生长周期的A549细胞,实验组共分为7个亚组,分别施加500~2 500V/cm的高压电场,对照组未施加电场处理。经生长抑制实验、黏附实验、侵袭及转移实验检验不同强度高压电场对A549细胞的影响。结果:1)细胞黏附能力:实验组各亚组的黏附细胞数明显少于对照组,实验组各亚组与对照组、实验组各亚组之间差异有统计学意义,P<0.001。2)细胞迁移能力:电场强度为500V/cm的实验亚组与对照组比较,差异无统计学意义,P=0.089;电场强度≥750V/cm的各实验亚组之间、及其与对照组比较,差异有统计学意义,P<0.001。3)细胞侵袭能力:电场强度为500和750V/cm的实验亚组与对照组比较,差异无统计学意义,P=0.181;电场强度≥1 000V/cm的实验亚组与对照组间比较,差异有统计学意义,P<0.001;电场强度为1 000~1 250V/cm的实验亚组和1 500~2 000V/cm实验亚组间比较,差异有统计学意义,P<0.001。结论:体外高压电场可以明显抑制A549细胞的黏附、迁移和侵袭等能力;随着电场强度的增加,肿瘤细胞的抑制程度也随之增加。
Objective: To investigate the effects of different intensities of high-voltage electric field on the adhesion, migration and invasion of lung cancer cell line A549. Methods: The A549 cells in the growth cycle were selected. The experimental group was divided into seven subgroups, and the high voltage electric field of 500-2 500V / cm was applied respectively. The control group was not treated with electric field. The growth inhibition, adhesion, invasion and metastasis experiments were conducted to examine the effects of different electric fields of high intensity on A549 cells. Results: 1) Adherence ability of cells: The number of adherent cells in each subgroup of experimental group was significantly less than that of control group, there was significant difference between each subgroup of experimental group and control group and each subgroup of experimental group (P <0.001.2) ) Cell migration ability: There was no significant difference between the experimental subgroups with electric field intensity of 500V / cm and the control group, P = 0.089; The electric field intensity ≥750V / cm between the experimental subgroups and their comparison with the control group , The difference was statistically significant, P <0.001.3) Cell invasion ability: There was no significant difference between the experimental subgroups with electric field intensity of 500 and 750 V / cm compared with the control group, P = 0.181; the electric field intensity≥1 000V / cm between the experimental subgroup and the control group, the difference was statistically significant, P <0.001; electric field intensity of 1 000 ~ 1 250V / cm experimental subgroups and 1 500 ~ 2 000V / cm experimental subgroups, the difference Statistically significant, P <0.001. CONCLUSION: In vitro high-voltage electric field can obviously inhibit the adhesion, migration and invasion of A549 cells. With the increase of electric field intensity, the inhibition degree of tumor cells also increases.