论文部分内容阅读
目的探讨弥散型和局限型脑干胶质瘤的病理学、超微结构特点以及增殖特性。方法收集18例脑干胶质瘤标本,应用苏木精-伊红染色观察18例脑干胶质瘤的形态变化,应用免疫组织化学方法检测10例脑干胶质瘤Ki-67蛋白表达,应用透射电子显微镜观察8例脑干胶质瘤细胞超微结构。结果苏木精-伊红染色结果显示:大多数弥散型脑干胶质瘤细胞生长活跃或具有恶性转化倾向。透射电子显微镜结果显示:弥散型脑干胶质瘤生长较局限型脑干胶质瘤活跃。免疫组化结果显示:所有肿瘤均表达Ki-67蛋白,弥散型脑干胶质瘤增殖指数明显高于局限型脑干胶质瘤(P<0.01)。结论弥散型脑干胶质瘤较局限型脑干胶质瘤生长活跃、细胞增殖快,可能与其恶性生物学行为有关。
Objective To investigate the pathology, ultrastructure and proliferative characteristics of diffuse and localized brain stem gliomas. Methods Eighteen cases of brain stem glioma were collected. The morphological changes of 18 cases of brain stem glioma were observed by hematoxylin-eosin staining. The expression of Ki-67 protein in 10 cases of brain stem glioma was detected by immunohistochemistry. Transmission electron microscopy was used to observe the ultrastructure of 8 glioma cells. Results Hematoxylin-eosin staining showed that most diffuse brain stem glioma cells grew actively or had a tendency of malignant transformation. Transmission electron microscopy revealed that diffuse brain stem glioma was more active than localized brain stem glioma. The results of immunohistochemistry showed that Ki-67 protein was expressed in all tumors, proliferation index of diffuse brain stem glioma was significantly higher than that of localized brain stem glioma (P <0.01). Conclusion Diffusion-type brain stem gliomas are more active than localized brain stem gliomas and proliferate rapidly, which may be related to their malignant biological behavior.