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目的探讨电刺激家兔中缝背核(DRN)对家兔脑皮质微血流的影响及其机理。方法应用氢清除法测定局部脑组织血流(rCBF)及观测软脑膜微循环。结果电刺激DRN后脑皮质rCBF减少44.2 %(P<0.01) ,软脑膜微动脉管径缩小 ,血流速度减慢 ,血流量减少 ,作用持续15min。切断颈交感神经或破坏蓝斑核(LC)后再刺激DRN ,rCBF分别减少15.8 %和16.6 %(P<0.05)。rCBF降低幅度均小于正常动物。电刺激DRN对半球脑血流动力学无显著影响。结论电刺激中缝背核可致家兔脑皮质微动脉收缩 ,局部组织血流减少。切断颈交感神经、破坏蓝斑核rCBF降低 ,但幅度小于正常动物。
Objective To investigate the effect and mechanism of electrical stimulation of dorsal raphe nucleus (DRN) on cerebral microcirculation in rabbits. Methods Local cerebral blood flow (rCBF) and pia mater microcirculation were measured by hydrogen scavenging assay. Results The rCBF of cerebral cortex decreased 44.2% (P <0.01) after electrical stimulation of DRN. The diameter of the pia mater microvessel decreased, blood flow decreased and blood flow decreased for 15 minutes. After cutting off the cervical sympathetic nerve or destroying the locus coeruleus (LC), the rCBF decreased by 15.8% and 16.6% (P <0.05) respectively. rCBF decreased less than normal animals. Electrical stimulation of DRN had no significant effect on hemodynamics of hemispheres. Conclusion Electrical stimulation of dorsal raphe leads to the contraction of arteriolar microvessels in rabbits and the decrease of blood flow in local tissues. Cervical sympathetic nerve transection, the destruction of locus coeruleus rCBF decreased, but less than the normal range of animals.