目的定性定量检测更年期综合征个体肠道微生物群落,探讨该状态中的人群肠道内微生态平衡状态及其特点,从而为研究更年期综合征人群的防治新策略提供数据资料。方法设患更年期综合征妇女研究组和相近年龄同性别的健康妇女对照组,采用光冈法检测相关肠道菌群(双歧杆菌属、乳杆菌属、类杆菌属、产气荚膜梭菌、消化链球菌属、肠杆菌科、肠球菌属及酵母菌)的菌群值,同时计算和比较双歧杆菌属细菌数量与肠杆菌科细菌数量的对数值比值(B/E值)。结果与相似年龄同性别的健康人群对照组比较,更年期综合征人群研究组肠道双歧杆菌数量显著降低(P<0.01);B/E值较显著减少(P<0.01);肠杆菌科细菌、消化链球菌、肠球菌数量显著增加(P<0.01,P<0.01,P<0.05)。结论更年期综合征人群肠道双歧杆菌数量显著减少,肠杆菌科及肠球菌细菌数量显著增加,益生菌群与肠杆菌科结构发生改变,可能更年期综合征人群由于神经内分泌失调等健康状态的下降,引起肠道有益菌群与腐败菌比例发生变化,从而引起肠道微生态失调。 OBJECTIVE: To qualitatively and quantitatively detect intestinal microflora in individuals with menopausal syndrome, and to explore the status of microecological balance and its characteristics in the intestine of the population, so as to provide data for studying new strategies for prevention and treatment of climacteric syndrome. Methods The women with climacteric syndrome and the control group of healthy women of same age and gender were enrolled. The intestinal flora (Bifidobacterium, Lactobacillus, Bacteroides, Clostridium perfringens , Peptostreptococcus, Enterobacteriaceae, Enterococcus and Saccharomyces) were calculated and compared. The logarithm value (B / E value) of the number of bacteria in the genus Bifidobacteria and the number of Enterobacteriaceae bacteria was calculated and compared. Results Compared with control group, the number of intestinal Bifidobacterium in the study group of menopausal syndrome was significantly lower (P <0.01), the B / E value was significantly decreased (P <0.01), the number of Enterobacteriaceae , Peptostreptococcus and Enterococcus were significantly increased (P <0.01, P <0.01, P <0.05). Conclusions The number of intestinal Bifidobacterium in the population of menopausal syndrome is significantly reduced, the number of Enterobacteriaceae and Enterococcus bacteria is significantly increased, the structure of probiotics flora and Enterobacteriaceae are changed, and the health status of menopause syndrome may decline due to neuroendocrine disorders , Causing the intestinal flora and spoilage bacteria ratio changes, causing intestinal micro-ecological disorders.