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利用换碱洗脱法,可以从家蚕蛹皮中提取到较高分子量的白色壳聚糖,脱乙酰度达91%以上;经H_2O_2降解,可制备从4.31×10~4~1.32×10~6间不同分子量的壳聚糖;不同分子量的壳聚糖可以制成相应的适宜载体用于酶的固定化,所得固定化酶具有较高的活力和较好的操作稳定性。
Using alkali-washing method, higher molecular weight chitosan can be extracted from the silkworm pupae skin, the degree of deacetylation is more than 91%. After H_2O_2 degradation, the white chitosan with higher molecular weight can be prepared from 4.31 × 10 ~ 4 ~ 1.32 × 10 ~ 6 Between different molecular weight of chitosan; different molecular weight of chitosan can be made into the appropriate carrier for enzyme immobilization, the resulting immobilized enzyme has high activity and good operational stability.