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目的探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)增殖的作用。方法根据文献从成年大鼠海马原代及传代培养NG2细胞,以免疫荧光染色法鉴定细胞性质,以乳酸脱氢酶(LDH)分析法测定细胞活性,以原位缺口末端标记技术(即TUNEL法)观察细胞凋亡,5’-溴脱氧尿嘧啶核苷(BrdU)掺入法鉴定新生细胞。结果CL处置明显增加NG2细胞数,并显著减少TUNEL阳性细胞数,但对BrdU阳性细胞数无明显影响。结论CL能抑制NG2细胞凋亡,从而促进NG2细胞的增殖。
Objective To investigate the effect of cerebrolysin (CL) on the proliferation of NG2 proteoglycan positive neural progenitor cells (NG2 cells). Methods According to the literature, NG2 cells were cultured from primary and subcultured hippocampus of adult rats. The cell characteristics were identified by immunofluorescence staining. The cell viability was measured by lactate dehydrogenase (LDH) assay. TUNEL method ) Were used to observe the apoptosis. BrdU incorporation assay was used to identify newborn cells. Results CL treatment significantly increased the number of NG2 cells, and significantly reduced the number of TUNEL positive cells, but no significant effect on the number of BrdU positive cells. Conclusion CL can inhibit the apoptosis of NG2 cells and promote the proliferation of NG2 cells.